Tips & Tricks For Successful Vitrification


A successful cryopreservation program has been a requirement of fertility clinics throughout the world. It is important to note that as “freeze all cycles are gradually making advances, the requirement of a successful cryopreservation strategy is the requirement of the day.

Vitrification is a popular option lately for cryopreservation . It may vary slightly depending on the temperature, cryo device and the brand of the media. However, the fundamental principle and procedure are the same in all these variables. The process of Vitrification is carried out by using the readily available Vitrification materials. The embryos are subjected to the ES (Equilibration media) and then into the VS (Vitrification media) prior to being loaded onto a cryo device and then sucked in liquid nitrogen. For an embryologist it has been observed for a long time that Vitrification is a lot more difficult to master than ICSI or other procedures. It is unfortunate that there isn’t an comprehensive learning program pertaining in the exact same. Fertility clinics do not follow an established training plan for Vitrification which may result in the operator’s incompetence with poor survival rates, and fluctuations in results!

Vitrification of oocytes

The final stage of embryo loading is the defining element of the entire process and the whole Vitrification program is built on the foundation of that vital final minute. Naturally, the focus will be on the last step , which involves tasks such as washing embryos using the Vitrification System, loading them up with little media onto the cryo device, then emptying the media of excess and dumping in liquid nitrogen. The step is not difficult but certainly is meticulous in assessing the microscopic aspects associated with it, along with the speed of the race.

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The greatest challenge with Vitrification is the mix of speed with time! However, the technique can be implemented successfully after an extensive and systematic method to master it through self-designing an ideal learning environment.

First of all, once you are familiar with the procedure simply learning the protocol isn’t the main thing. There has to be an knowledge of the research behind it. It is common to see people panicking when they see embryos shrinking in ES!

In the beginning it is recommended that embryologists to be knowledgeable about all of the elements of a Vitrification system. The objective here is to complete the task without time constraints. The final step could be broken into mini steps to allow for better adaptation. Embryologist can learn to adjust the coarse knob on the microscope to select the embryos after they have afloat due to the change in viscosity within VS. This includes the concentration of the cryotop in the microscope, the positioning of the hand, learning about the washing process, and ultimately, the ability to load embryos!

After you’ve accomplished the task, time will come into the picture. The use of a stop watch and practicing loading the required amount of time becomes the primary focus. At first, loading time could be longer than two minutes. After a few weeks of practice you’ll decrease the amount of time required and get yourself into the interval of 60 to 90 seconds! The practice can be performed by using oocytes that have been degenerated.

Fourthly, after you’ve taken care of the time then the next step is to survive! This is the time when you’ll use embryos from discarded ones and then freeze them as if you are performing the actual procedure. In this case, the ES will come into the practice and you can observe the intricate details like the reactions of embryos and media. At this point, the thawing technique will also be incorporated as it too contributes in the general survival. The results can be recorded, and will give an insight into the overall improvement of the worker.

Finally, once you are completed with the embryos’ survival It is recommended to freeze embryos to be used for donors since an ED cycle has the best embryos of high-quality. Blastocysts should be considered more in the beginning phase of freezing. They are more durable than say day two or day three embryos. This can be furthered by increasing the number of cryo machines gradually and evaluating the performance of previously frozen cryo devices concurrently.

This extensive method of learning Vitrification may take up to six months total, contingent on the cycle that is taking place in fertility clinics, which certainly affects the availability of games and resources(such as expired media for practicing). It could help improve proficiency over the technique and can be standardized and rigorously implemented in a working fertility center itself. Vitrification opens the way for embryos to hibernate during an ice age , and perhaps we can make their journey more easy!

Learn process of vitrification of embryos, Advanced course in cryopreservation, IVF training for Gynaecologist and Embryologist, Hands-on IVF IUI ICSI training courses and how to set up IVF and IUI Laboratory in India for Gynaecologist and Embryologist at 

Embryology Academy for Research & Training is an IVF training institute in the field of assisted reproduction technologies which offers Short course and hands on training to young aspirants and embryologists. We at EART with our judicious mixture of various teaching modules that include lectures, practical hands-on-training and demonstrations will provide you with a high-quality education which is to your complete satisfaction. At EART our goal is to offer the most advanced and competitive training available on the area of ART. Our customized training program since its start in the year 2003 has given us the satisfaction of training 150 plus national and international applicants. EART is a reputable research institute for IVF Training programs for IVF or ICSI/ART Micromanipulation, Reproductive Medicine.


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